Sleep disturbances among medical workers during the outbreak of COVID-2019.

Sleep disturbances among medical workers during the outbreak of COVID-2019.

The outbreak of CoronaVirus Illness-2019 (COVID-19) has posed unprecedented strain and threats to healthcare staff in Wuhan and your complete nation.To evaluate the impact of the COVID-19 outbreak on the sleep high quality of healthcare staff in a kids’s healthcare centre in Wuhan.A cross-sectional, anonymized, self-reported questionnaire survey was performed on the Kids’s Healthcare Centre of Renmin Hospital, Wuhan College, Wuhan, China.

The questionnaire consisted of three elements, together with socio-demographic traits and COVID-19 epidemic-related elements, the Pittsburgh sleep high quality index (PSQI), and Zung’s self-rating nervousness scale (SAS) and self-rating melancholy scale (SDS).In complete, 47 out of 123 (38%) individuals with PSQI scores > 7 have been recognized as having sleep disturbance.

A logistic regression evaluation confirmed that sleep disturbance was independently related to being an solely little one (adjusted odds ratio (OR) and 95% confidence interval (CI) 3.40 (1.21-9.57), P < 0.05), publicity to COVID-19 sufferers (adjusted OR and 95% CI 2.97 (1.08-8.18), P < 0.05) and melancholy (adjusted OR and 95% CI 2.83 (1.10-7.27), P < 0.05).We noticed that, throughout the outbreak of COVID-19, sleep disturbance was extremely prevalent amongst paediatric healthcare staff, and sleep disturbance was independently related to being an solely little one, publicity to COVID-19 sufferers and melancholy.

Subsequently, extra psychological well being companies are required for front-line paediatric healthcare staff in Wuhan.

The medical and immunological options of pediatric COVID-19 sufferers in China.

In December 2019, the coronavirus illness 2019 (COVID-19) brought on by novel coronavirus (SARS-CoV-2) emerged in Wuhan, China and quickly unfold worldwide. Few info on medical options and immunological profile of COVID-19 in paediatrics.

Sleep disturbances among medical workers during the outbreak of COVID-2019.
Sleep disturbances amongst medical staff throughout the outbreak of COVID-2019.

The medical options and therapy outcomes of twelve paediatric sufferers confirmed as COVID-19 have been analyzed. The immunological options of youngsters sufferers was investigated and in contrast with twenty grownup sufferers. The median age was 14.5-years (vary from 0.64 to 17), and 6 of the sufferers have been male.

The common incubation interval was Eight days. Clinically, cough (9/12, 75%) and fever (7/12, 58.3%) have been the commonest signs. 4 sufferers (33.3%) had diarrhea throughout the illness. As to the immune profile, kids had increased quantity of complete T cell, CD8+ T cell and B cell however decrease CRP ranges than adults (P < 0.05).

Floor-glass opacity (GGO) and native patchy shadowing have been the standard radiological findings on chest CT scan. All sufferers acquired antiviral and symptomatic therapy and the symptom relieved in 3-Four days after admitted to hospital.

The paediatric sufferers confirmed gentle symptom however with longer incubation interval. Kids contaminated with SARS-CoV-2 had completely different immune profile with increased T cell quantity and low inflammatory elements stage, which could ascribed to the gentle medical symptom.

We advise that nucleic acid check or examination of serum IgM/IgG antibodies towards SARS-CoV-2 must be taken for youngsters with publicity historical past no matter medical symptom.

Prediction of the COVID-19 spread in African countries and implications for prevention and control: A case study in South Africa, Egypt, Algeria, Nigeria, Senegal and Kenya.

Prediction of the COVID-19 spread in African countries and implications for prevention and control: A case study in South Africa, Egypt, Algeria, Nigeria, Senegal and Kenya.

COVID-19 (Corona Virus Illness 2019) is globally spreading and the worldwide cooperation is urgently required in joint prevention and management of the epidemic.

Utilizing the Most-Hasting (MH) parameter estimation technique and the modified Vulnerable Uncovered Infectious Recovered (SEIR) mannequin, the unfold of the epidemic below three intervention eventualities (suppression, mitigation, mildness) is simulated and predicted in South Africa, Egypt, and Algeria, the place the epidemic conditions are extreme.

The research are additionally carried out in Nigeria, Senegal and Kenya, the place the epidemic conditions are rising quickly and the socio-economic are comparatively under-developed, leading to extra difficulties in stopping the epidemic.

Outcomes indicated that the epidemic will be principally managed in late April with strict management of state of affairs one, manifested by the circumstance within the South Africa and Senegal.

Below reasonable management of state of affairs two, the variety of contaminated folks will improve by 1.43-1.55 instances of that in state of affairs one, the date of the epidemic being managed can be delayed by about 10 days, and Algeria, Nigeria, and Kenya are in accordance with this case.

Within the third state of affairs of weak management, the epidemic can be managed by late Could, the entire variety of contaminated circumstances will double that in state of affairs two, and Egypt is consistent with this prediction.

In the long run, a sequence of epidemic controlling strategies are proposed, together with affected person quarantine, shut contact tracing, inhabitants motion management, authorities intervention, metropolis and county epidemic threat degree classification, and medical cooperation and the Chinese language help.

Prediction of the COVID-19 spread in African countries and implications for prevention and control: A case study in South Africa, Egypt, Algeria, Nigeria, Senegal and Kenya.
Prediction of the COVID-19 unfold in African international locations and implications for prevention and management: A case examine in South Africa, Egypt, Algeria, Nigeria, Senegal and Kenya.

Simpler methods are required to strengthen public consciousness of COVID-19: Proof from Google Tendencies.

The outbreak of coronavirus illness 2019 (COVID-19) has posed stress on the well being and well-being of each Chinese language folks and the general public worldwide. World public curiosity on this new situation largely displays folks’s consideration to COVID-19 and their willingness to take precautionary actions.

This examine aimed to look at international public consciousness of COVID-19 utilizing Google Tendencies.StrategiesUtilizing Google Tendencies, we retrieved public question knowledge for phrases of “2019-nCoV + SARS-CoV-2 + novel coronavirus + new coronavirus + COVID-19 + CoronaVirus Illness 2019″ between the 31st December 2019 and the 24th February 2020 in six main English-talking international locations, together with the USA, the UK, Canada, Eire, Australia, and New Zealand. Dynamic sequence evaluation demonstrates the general change development of relative search quantity (RSV) for the subject on COVID-19. We in contrast the top-ranking associated queries and sub-regions distribution of RSV about COVID-19 throughout totally different international locations.

The correlation between day by day search volumes on the subject associated to COVID-19 and the day by day variety of folks contaminated with SARS-CoV-2 was analyzed.

OutcomesThe general search development of RSV concerning COVID-19 elevated throughout the early interval of observing time and reached the primary apex on 31st January 2020.

A shorter response time and an extended period of public consideration to COVID-19 was noticed in public from the USA, the UK, Australia, and Canada, than that in Eire and New Zealand. A barely optimistic correlation between day by day RSV about COVID-19 and the day by day variety of confirmed circumstances was noticed (P < 0.05). Folks throughout international locations introduced a varied curiosity to the RSV on COVID-19, and public consciousness of COVID-19 was totally different in varied sub-regions inside international locations.

The outcomes counsel that public response time to COVID-19 was totally different throughout international locations, and the general period of public consideration was brief. The present examine reminds us that governments ought to strengthen the publicity of COVID-19 nationally, strengthen the general public’s vigilance and sensitivity to COVID-19, inform public the significance of defending themselves with sufficient precautionary measures, and at last management the unfold of COVID-19 globally.

HilyMax transfection reagent: also effective with HL-1 cells

Dojindo’s HilyMax transfection reagent, distributed by the Microtech company, was tested by Dr. Yuri D’Alessandra, a researcher at the Immunology and Functional Genomics laboratories of the Monzino Cardiology Center in Milan.

The HilyMax reagent test on HL-1 cells
The HilyMax transfection reagent test performed on immortalized tumor cell lines (HeLa), widely used in scientific research, and on atrial cardiomyocytes (HL-1) used for the study of many human heart diseases, has given significant and satisfactory results compared to the results obtained with other transfect agents. HeLa and HL-1 cells were seeded in 24-well plates, following the Dojindo protocol and transfected with 0.5 micrograms / well of eGFP pCDNA 3.1 vector (containing a human gene of interest).

Results beyond all expectations
Since a very weak green fluorescence signal was detected by fluorescence microscopy, the evaluation of the expression of the GFP and the mRNA of the gene of interest was made by RT-qPCR. Both HeLa and HL-1 showed a very strong expression of the gene of interest. It should be emphasized that, by transfecting a human ORF, it was possible to determine that 100% of the levels of gene expression detected were due to the success of the transfection. The experiment was performed a second time with HL-1 cells in triplicate with overlapping results.

No previous attempt to transfect HL-1 cells with transfectants or electroporation has yielded comparable results.

Contamination of RNA samples from genomic DNA

To extract RNA from biological samples, specific reagents and kits based on the use of columns (spin column) are used. No RNA isolation method, however, ensures that RNA is free of genomic DNA contamination (gDNA). The presence of gDNA in RNA samples represents a problem when RNA must then be used in applications such as RT-PCR and qRT-PCR. False positives and alterations in the expression of genes are the result of a use of RNA contaminated with gDNA. Hence the need to eliminate gDNA contamination from RNA samples. In vitro digestion with deoxyribonuclease I (DNase I) is the most effective method for removing gDNA contamination in RNA samples. Unfortunately, the removal or inactivation of this enzyme after digestion is problematic.

The most commonly used methods for inactivating the enzyme (thermal inactivation, chelating agents such as EDTA, treatment with proteinase K followed by extraction with phenol / chloroform) are ineffective and even harmful to the integrity of RNA, in addition the fact that large quantities of RNA are lost (think of when you have available small quantities of RNA obtained from small biological samples). An alternative is represented by the use of DNase directly on the spin column, although this method is not efficient for samples with large quantities of DNA, such as spleen and thymus for which in vitro treatment is required.

Abmgood’s AccuRT Genomic DNA Removal Kit is based on a very simple and quick method (10 min) and effectively eliminates gDNA without loss or degradation of RNA. Thermal inactivation of the enzyme is not required. The treated RNA is compatible with various applications such as RT-PCR, qRT-PCR, microarray, Northern and more.

The new human coronavirus

Disease & Contagion
The new human coronavirus, severe acute respiratory syndrome coronavirus (SARS-CoV-2), belongs to the species of Severe Acute Respiratory Syndrome related Coronavirus, genus beta-coronavirus, subgenus Sarbecoviruses, lineage B (Zhou 2019) . Coronaviruses cause respiratory infections, sometimes with an enteral component, in humans and animals. This group also includes SARS-CoV, which caused outbreaks in 2003 in various regions of the world.

The new coronavirus (SARS-CoV-2) is genetically most closely related to the SARS coronavirus. Like SARS, it uses the ACE2 receptor. This occurs, among other things, on the alveolar epithelium, which is seen as the explanation for the predilection of SARS for replication in the low airways (Haagmans 2020 ).

Incubation period
2-14 days (average 5-6 days).

Disease symptoms
Varying, from mild nonspecific respiratory complaints: colds; sore throat (14%); (dry) cough ( 68%); fatigue (38%); sputum production (33%); muscle and joint pains (15%) ; headache (14%) and increase, to more serious syndromes with fever (> 38 degrees Celsius, 88%); shortness of breath (19%) and pneumonia , up to acute respiratory stress syndrome and septic shock. Also reported (in a smaller proportion of patients): diarrhea (4%) ; nausea and vomiting (5%). About 80% of the reported cases have mild to moderate complaints, 13.8% had serious complaints and 6.1% had very serious complaints.

The patients with complications are divided into ‘severe pneumonia’ if they are in need of oxygen (about 65% of the cases), ‘critical’ if they need ventilation (about 20%), or ‘fatal’ (about 15% of the patients with pneumonia).

China reported a case fatality rate of 2.3% in March 2020. Both the severity, course and case fatality rate depend on underlying conditions and increase in the elderly above 70 years.

(Guan 2020, Huang 2020, Wang 2020, Haagmans 2020, ECDC 2020a)

Disease symptoms in pregnancy
There are no indications that a COVID-19 infection is different in a healthy pregnant woman than in a non-pregnant woman. But like some other viral respiratory infections, complications such as pneumonia and fever in a pregnant woman can be serious. This is especially true for the third trimester (> 28 weeks) of pregnancy, due to the mechanical limitation of the growing abdomen resulting in a reduction in lung capacity. This therefore applies not only to COVID-19 infections but also to other respiratory infections.

Natural immunity

Natural immunity in pregnancy

Not applicable. It is assumed that a zoonotic source (bats and / or pangolins) ( Wang 2020).

Contamination path
The disease is transmitted from person to person.

Straight away
Drip infection: transmission via large drops from coughing and sneezing within a distance of 1.5 meters. Via aerosols during aerosol-forming procedures (tracheal intubation, non-invasive ventilation, tracheostomy, cardiopulmonary resuscitation, manual manual ventilation prior to intubation, bronchoscopy, tracheostomy procedures, suctioning) ( WHO 2020a).

There is no evidence that the virus spreads aerogenically (via airborne particles), except through aerosols during aerosol-forming procedures (tracheal intubation, non-invasive ventilation, tracheostomy, cardiopulmonary resuscitation, manual manual ventilation prior to intubation, bronchoscopy, operations on the tracheostomy, suction) ( WHO 2020a).

Perinatal transmission
There is no evidence of perinatal transmission.

As with other viral respiratory infections, transmission of the virus through breastfeeding is unlikely to play a role. Good hand and cough hygiene while breastfeeding is important. If the mother coughs a lot, you can choose to have expressed milk given by another parent / caregiver.

Contagious period
Exact data about the contagious period is missing. In any case, a patient is contagious during the symptomatic phase ( Huang 2020 ). Both patients with mild and severe complaints can excrete virus (Zhang 2020). The virus can also be detected by PCR in the patient’s faeces ( WHO 2020b, ECDC 2020b). After the symptoms have disappeared, the virus can still be detected by PCR in both the throat (7-14 days) and faeces (4-5 weeks). Virus has also been detected in the throat by PCR 1-2 days before symptoms appear (Tong 2020). In some asymptomatic individuals, the virus can be detected in the throat by PCR without becoming ill themselves later (Bai 2020, Hoehl 2020, Pan 2020, Zou 2020).

It is unclear whether the detection of virus in asymptomatic persons is related to infectivity. Based on the current literature, it is mainly symptomatic persons who contribute to the spread and the contribution of the asymptomatic persons to the spread seems to be limited (Ghinai 2020). Only one asymptomatic person with a virus detected in the throat by PCR was able to actually breed the virus ( Hoehl 2020). Also, there are currently no indications that faeco-oral transmission contributes to the spread.

Exact data on the infectiousness are missing. The sicker someone is, the more virus the person can spread. Outside the body, the virus can only survive for a short time. How long that is exactly is still unknown. This can vary from a few hours to a few days. This depends on, for example, the type of surface, the temperature and the humidity. There is no evidence that people have been infected by this ( ECDC 2020b). Until now it has not been possible to establish that contaminated surfaces play a role in the spread (Moriarty 2020).

Perinatal transmission
To date, no intrauterine transmission or an increased risk of miscarriage or birth defect from infection with the new coronavirus has been described. Perinatal transmission has been described (Liu 2020, Mullens 2020).

Direct diagnostics
Detection of the virus can be done with (real-time) reverse transcription (RT) -PCR for detection of viral RNA . Obviously, for optimal detection of the virus, regardless of the technique, the sample collection – nasopharynx (nose) some and oropharynx (throat) some – should be done correctly. SARS-CoV-2 is more detectable in nasopharynx smears than in oropharynx smears in patients with COVID-19. It remains important to decrease both, as there are patients who are positive only in one of the two locations. Therefore, for SARS-CoV-2 diagnostics, always send in a nasopharynx watts, in addition to an oropharynx watts and, if possible, a sputum sample or bronchoalveolar lavage fluid (Yang 2020).

To save material, it is possible to sample both nose and throat with one stick, send two sticks in one tube with shipping medium, or test materials pooled. For explanation, see the appendix Additional diagnostic information .

The PCR assays used for SARS-CoV-2 to date target two targets: the E gene and the RdRP gene. With the experience gained so far, testing for the E gene can only be considered. The amplification curve should be well assessed (especially at a Ct> 30). If the curve is abnormal, unreliable or difficult to interpret, or if there is an epidemiologically unexpected positive, for example the first case on one of the Caribbean islands, confirmation is necessary. Depending on the sensitivity of the local implementation, this can be done with the RdRP PCR , by retesting the same sample, or by resampling the patient. Also, false positive signals from primers and probe that may be contaminated with synthetic E gene run control should still be monitored. A comprehensive entry check therefore remains crucial (Corman 2020).

Collection technique and protective measures
For collection technique, see the appendix Collection technique specific viral diagnostics .

For hygiene measures during acceptance, see Generic scenario , appendix 8: ‘Hygiene measures’, section ‘Protection during sampling’ (page 2).

Executive laboratories
In addition to Erasmus MC and RIVM-IDS, there are 13 regional upscaling laboratories where diagnostics can be performed to SARS-CoV-2 . In addition, since the beginning of March, other medical microbiological laboratories have started or are implementing diagnostics to SARS-CoV-2. An urgent request is being made to all GGDs to send the samples to the upscaling laboratory in their OWN region.

For an overview of the relevant laboratories and for additional information about accessibility, transport, laboratory safety and sampling materials, see the Appendix Additional diagnostics information .

Indirect diagnostics
Serology is not (yet) available, but research is being conducted into the usefulness of serological tests. If patient’s serum is available, the applicant is requested to send it along with the other samples. If a serological test is not yet available, this does not directly contribute to diagnostics for the acute phase, but to subsequent imaging of the COVID-19. Serum samples can be sent to RIVM-IDS and / or Erasmus MC .

COVID-19 Resume


Pathogen: Coronavirus  SARS-CoV-2
Contamination route: Human-to-human transmission via direct drip infection (coughing and sneezing) and aerosols during aerosol-forming operations
Incubation time: 2-14 days (average 5-6 days)
Infectious period: Not yet fully known. At least during the symptomatic phase, after which the virus can be detected in the throat / faeces for longer by PCR
Measures: Reporting obligation group A; source and contact research; insulation and further measures on indication
Symptoms : Mild respiratory complaints with fever to severe pneumonia and dyspnea

This CONCEPT guideline is intended for GGD ‘and and hospitals and indicates how to treat a patient with (suspected) COVID-19. This information is regularly updated; refreshing the app and web page before use is recommended.

COVID-19 detection rapid tests structure
COVID 19 positive Rapid test

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